Composite

Part:BBa_I20300

Designed by: Francois St-Pierre   Group: Endy Lab   (2010-06-21)

RBS from T7 gene 4, optimal aligned spacing + Z0301 venus YFP

A composite of: (1) BBa_I20299, a strong RBS derived from phage T7 gene 4, and (2) BBa_Z0301, a venus YFP codon-optimized for E.coli expression

Note there are no Biobrick "scar" between the RBS and the fluorescent protein

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 167
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 218


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Categories
Parameters
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